Denitrification Mechanism of Investigation Chaetomium Elatum on Nitrocellulose

doi:10.12382/bgxb.2022.0832

Abstract

Abstract:

In view of the serious potential safety problems of waste propellants, five microorganisms that can reduce the nitrogen content of nitrocellulose (NC) are obtained by sampling, enriching, domesticating, separating and purificating near and around the wastewater treatment tank of nitrocellulose production. The time optimization experiment was carried out on the chaetomium elatum which has the best denitrification effect among them. It is found that the nitrogen content of NC could be reduced from 13.06% to 10.83% over time, and the X-ray photoelectron spectroscopy(XPS) results show that the content of N element on the surface of NC gradually decreases. Fourier Transform infrared spectroscopy (FT-IR), inVia Raman microscope (Raman) and gel permeation chromatography (GPC) are used to characterize the structure and molecular weight of NC. The infrared spectrum shows that the —OH peak intensity of NC at about 3400cm^-1 is gradually increased because the hydrolysis of chaetomium elatum and O—NO₂ occurs and the Raman spectrum shows that the intensity of NC energetic groups (O—NO₂) decreases significantly. At the same time, it is found by GPC that the number-average molecular weight and weight-average molecular weight of NC could be reduced by 2.34% and 6.40%, respectively, due to chaetomium elatum. In order to further determine the biological enzymes acting on NC, the separation of extracellular enzymes and intracellular enzymes, and ammonium sulfate gradient precipitation experiments proved that the extracellular enzymes precipitated by 0-30% (NH₄)₂SO₄ could effectively reduce the nitrogen content of NC. The significant effect of chaetomium elatum on reducing the nitrogen content of NC makes it have broad application prospects in the reuse of waste military NC and the biological denitrification of propellants.

Key words: nitrocellulose, chaetomium elatum, nitrogen content, molecular structure, biological denitrification

CLC Number:

TQ562

HUANG Juan, XUE Huihui, ZHANG Alei, CHEN Kequan, ZHOU Jie, DING Yajun, XIAO Zhongliang. Denitrification Mechanism of Investigation Chaetomium Elatum on Nitrocellulose[J]. Acta Armamentarii, 2024, 45(3): 875-884.

Figures/Tables 11

Fig.1 Co-culture reaction and reextraction process of NC and microorganism

Fig.2 Denitrification microorganisms for NC

Fig.3 Changes of nitrogen content in NC treated by microorganisms

Fig.4 The full-scale XPS survey spectra of NC

Fig.5 The high resolution XPS analysis curves of NC obtained in the areas of N 1s at different degrees of denitrification by chaetomium elatum

Fig.6 Infrared spectra of NC before and after denitrification by chaetomium elatum

Fig.7 Raman spectra of NC before and after denitrification by chaetomium elatum

Fig.8 Raman line sweep spectra of NC with different denitrification degrees of chaetomium elatum

Fig.9 Changes of relative molecular mass distribution of nitrocellulose before and after denitrification by chaetomium elatum

Fig.10 Denitrification of NC by intracellular and extracellular enzymes

Fig.11 Denitrification degree of NC by protein precipitated by ammonium sulfate

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